Dose of 150 mg/kg of the freshly prepared solution was administered intraperitoneally to 16 hr fasted mice. A frost hardy, low growing plant that has a spreading habit - not unlike the strawberry - by sending out runners to make leafy mat out of which grow spikes of blue flowers in spring. Hence, it can be concluded that Ajuga integrifolia, especially at 200 mg/kg and 400 mg/kg of bodyweight of both the crude extract and aqueous solvent fraction, is remarkably effective against STZ-induced diabetic mice models as well as in the oral glucose loaded mice models, thereby validating its ethnomedicinal usage. Weight loss of the mice is often associated to STZ and hyperglycaemic complication [22]. is an accepted name This name is the accepted name of a species in the genus Ajuga (family Lamiaceae). Chú thích Liên kết ngoài. Learn more about Ajuga Nipponensis uses, effectiveness, possible side effects, interactions, dosage, user ratings and products that contain Ajuga Nipponensis Ajuga integrifolia has a wide native range, from northeast Africa, through Arabia, temperate and tropical Asia to New Guinea. According to between groups analysis, it was observed that 100 mg/kg AIRE did not improve bodyweight at the7th day and14th day of treatment compared to the diabetic control, but at 200 mg/kg and 400 mg/kg, AIRE improved significantly the bodyweight of the mice () at the 7th day and () at the 14th day of treatment compared to the diabetic control. Overnight fasted STZ-induced diabetic mice and normal mice were grouped into 6 groups (5 groups of mice having diabetes and 1 group of normal mice, 6 mice in each group) at random. Swiss albino male mice which were healthy weighed 20–30 g and aged 8–12 weeks were used in the study, and healthy female mice having similar weight and age to males were used for the acute oral toxicity study. Drugs and chemicals used in the study were streptozotocin (Sigma Aldrich, Germany), methanol absolute (Nice Chemical, India), glibenclamide (Julphar pharmaceutical, Ethiopia), trisodium citrate dehydrate (Blulux Laboratories, India), citric acid monohydrate (Lab Tech chemical, India), 40% glucose solution (Reyoung Pharmaceutical, China), sterilized water for injections (Nirman Ltd., India), and distilled water, whereas the instruments used in this study were analytical balance, pH meter, glucometer and test strips (Alliance international, Taiwan), beakers, Whatman filter paper No.1, funnels, glass rod, measuring cylinder, vacuum pump, spatula, pipettes, gavage (oral feeding syringe), animal cages, insulin syringe with needle, oven, and desiccators. These phytochemicals include phenolic compounds, alkaloids, terpenoids, flavonoids, tannins, and sterols among others. Acclimatization of the mice to the laboratory conditions was performed for 1 week prior to the start of the experiment. A large number of compounds have already been isolated from A. remota , including ergosterol-5,8-endoperoxide ( 6 ), ajugarin-I ( 1 ), 8- O -acetylharpagide ( 5 ) and several phytoecdysteroids. Ajuga integrifolia is an herb belonging to Lamiaceae family under genus Ajuga and is locally known as “Tut Astel” and “Akorarach” in Amharic and “Harmegusa” in Oromifa in various parts of Ethiopia [9–11]. F. Assefa, “Antidiabetic activity of ajuga remota benth (harmegusa) leaves in streptozotocin induced diabetic rats,” Addis Ababa University, Addis Ababa, Ethiopia, 2013, Thesis. Mice were screened for diabetes after 3 days of STZ injection, and fasting BGL > 200 mg/dl was included in the study as diabetic mice [25, 26]. Antioxidants are known to prevent oxidative stress, thereby treating DM and its complications [14–16]. Sign up here as a reviewer to help fast-track new submissions. (14) • Antimalarial: Study evaluated in vivo activity of crude water extracts of Ajuga remota Benth against Plasmodium berghei in mice. This member of the mint family spreads fast and can become invasive. The mice were stayed in light and dark cycle (12 hours of each cycle) which is a standard condition for laboratory animals and permitted open access to standard pellet diet and water ad libitum. This blood glucose reduction after 2 hours may be due to the glucose lowering activity of AIRE augmented by metabolic utilization of the already consumed glucose. Inductions of diabetes were performed by using streptozotocin (STZ). According to the results of the first mouse, another four female mice were recruited and fasted for 4 hours and then given a single dose of 2 g/kg and was observed rigorously in the same manner. It causes pancreatic β-cell destruction via DNA alkylation and strand breakage, thereby causing diabetes mellitus [36, 37]. In North Africa, plants of the genus Ajuga are used to treat diabetes and hypertension, as a panacea (cure-all), specifically for gastrointestinal disorders, and as an anthelmintic [ 10 ]. Long-term damage and failure of the eyes, kidneys, nerves, heart, and blood vessels were evident in chronic hyperglycemia [2]. value < 0.05 was considered statistically significant. No toxicity incidences have been reported related to Ajuga integrifolia in particular and the genus Ajuga in general so far [24, 33–35]. This was validated after 72 hours of STZ injection with sustained hyperglycemia. Group 1 was diabetic control treated with 10 ml/kg DW; doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg root extract of plant were given to experimental groups 2, 3, and 4, respectively; group 5 was diabetic positive control treated with 5 mg/kg glibenclamide, whereas group 6 was normal control treated with 10 ml/kg DW. An easy way to keep ajuga bugleweed in check is to surround your garden beds with it. The extract was more appreciably soluble in water than in organic solvent producing yields of hexane (12%), chloroform (17%), aqueous (69%), and remaining olegenous residue (2%). Then, each mouse’s BGL was determined at 1 hr, 2 hr, 4 hr, and 6 hr posttreatment [23, 24]. Entry for AJUGA bracteosa Wall. Then, blockage of KATP results in the influx of Ca2+ to cells which, in turn, cause depolarization in the cytosol with subsequent insulin secretion [39]. In addition, the roots of Ajuga integrifolia have larger amounts of chromium which may be correlated to its use as treatment for DM [18]. Working to the all cases, group 1 used as negative control was treated with 10 ml/kg distilled water (DW). It is not scientifically validated for its antidiabetic activity previously. It was also found that groups treated with the Ajuga integrifolia at the doses of 200 mg/kg and 400 mg/kg showed significant () bodyweight improvement at the 14th day of treatment compared to the respective baseline bodyweight, and the diabetic control group showed significant () reduction in bodyweight at the 14th day compared to the baseline. In this study, there was blood glucose lowering brought about by GLC-induced secretion of insulin from pancreatic β-cells; this indicates that a single dose of STZ at 150 mg/kg could not cause absolute destruction of β-cells. The plant will thrive in both shady and sunny locations. According to the result of the acute oral toxicity study, the lower, medium, and high doses of the plant extracts were determined. 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